The certified seedlines and rulebook are now one combined booklet and can be found here. Pmtv was first reported in britain in 1966 and subsequently discovered in cooler areas of europe, in the andes of south america, and in asia. Pomovirus is a genus of viruses, in the family virgaviridae. Distribution and diversity of potato moptop virus in sweden. Pdf potato moptop virus pmtv is a plant pathogenic virus that affects.
Screening of potato cultivars to potato moptop virus in. Reaction of potato varieties topotato moptop virus infection in the. Detection of potato mop top virus and tobacco rattle virus using a multiplex realtime fluorescent reversetranscription polymerase chain reaction assay. Elisa sets are supplied as 100, 300, 500 and for many articles as and 5000 test units. Apr 24, 2008 besides being a pathogen, powdery scab spongospora subterranea wallr. Established in 1937, the idaho potato commission ipc is a state agency that is responsible for promoting and protecting the famous grown in idaho seal, a federally registered trademark that assures consumers they are purchasing genuine, topquality idaho potatoes. Potato moptop virus umaine extension the university of maine. Potato moptop virus pmtv00overview eppo global database. Planting healthy seed potatoes is a key factor in maximizing the production of usable potatoes for consumption or processing. Loewe was established in 1988 as an independent, privately owned company. Johnson, extension crops specialist, university of maine cooperative extension. There are currently four species in this genus including the type species potato mop top virus. Jul 19, 20 potato mop top virus an emerging problem in the columbia basin jim crosslin, launa hamlin, rich quick, and chuck brown usdaars, prosser, wa a potato pathogen that is increasing in importance in the columbia basin is potato mop top virus pmtv.
Rtpcr results showed that the symptomatic tuber produced a band at 416 bp with the pmtv primers, which. This is a significant cause of rejections of both ware and processing crops. Unless stated otherwise the elisa reagents are optimized for a test volume of 200 l per well, ensuring the highest accuracy, repeatability and sensitivity of the elisa assay. Potato moptop virus pmtv was detected in potatoes grown in the united states and canada during surveillance testing by a reverse transcription. Gudmestad department of plant pathology, north dakota state university, fargo 58108. Potato mop top virus pmtv is a plant pathogenic virus transmitted through the vector spongospora subterranea that affects potatoes. Detection of potato viruses x and y in sap extracts by a modified indirect. General information about potato mop top virus pmtv00 eppo global database.
Once established in fields, the virus can survive in the. Because the virus is not passed to all the progeny tubers of an infected potato plant, it is gradually selfeliminating in potato stocks grown on virus free soil. Wisconsin department of agriculture, trade and consumer protection wisconsin pest survey fact sheet potato mop top virus. The unexpected detection of potato mop top virus pmtv in potatoes growing at several locations in the u. Nov 19, 2019 occurrence of potato mop top virus in japan. Pmtv is one of two viruses causing spraing in potato.
It is an important disease in the potato growing areas of the world. Use this page to stay up to date with the response to pmtv. Analysis of potato moptop virus survival probability in post. Potato virus x potato virus s potato virus y potato virus a potato virus m potato leaf roll virus tobacco rattle virus potato mop top virus potato virus v potato virus t andean potato latent virus andean potato mottle virus arracacha virus potato black ringspot virus tomato spottled wilt virus. Pmtv is in the viral genus pomovirus and is a furovirus fungustransmitted, rodshaped virus. Possible validation of rt pcr n and c europe peru affect tuber quality fungi potato yellow vein virus pyvv order. Detection of potato mop top virus and tobacco rattle virus. Elisa sets kits and controls loewe biochemica gmbh.
Infection is invariably linked with transmission by spongospora subterranea, the cause of powdery scab. The united nations economic commission for europe unece. Potato mop top virus pmtv was detected in potatoes grown in the united states and canada during surveillance testing by a reverse transcriptionpolymerase chain reaction rtpcr targeting the. As one of the commercial pioneers in the plant disease detection sector, we are offering a comprehensive range of diagnostic tools for the detection of plant diseases. The behaviour of potato mop top virus in soil, and evidence for its transmission by spongospora subterranea wallr. Virus description and symptoms potato mop top virus pmtv is a pomovirus, and is transmitted by the pathogen a protozoan with zoospores for infection which causes powdery scab disease, spongospora subterranea. One set is composed of the corresponding amount of igg and apconjugate solution. Symptoms induced by potato mop top virus in potato resemble those caused by tobacco rattle virus, although most potato cultivars react somewhat differently to the two viruses. Infections are commonly associated with tuber internal blemishing see also trv. Mar 16, 2014 potato mop top virus pmtv, which appears to be increasing in importance in the united states, is a soilborne virus. Potato moptop virus pmtv is a plant pathogenic virus transmitted through the vector spongospora subterranea that affects potatoes. Nov 22, 2019 serological relationship between potato paracrinkle virus, potato virus s and carnation latent virus.
Industry research articles idaho potato commission. Potato moptop virus is seed and soilborne, and vectored by spongospora subterranea f. Moptop virus, relationship to its vector springerlink. Detection of potato mop top virus in soils and potato tubers using baitplant bioassay, elisa and rtpcr. Reaction of potato varieties to potato moptop virus. Primerdesign ltd tm potato moptop virus genesig easy kit.
The use of tissue cultures to produce virus free clones from infected potato varieties. Detection of potato moptop virus in soils and potato tubers. The unexpected detection of potato moptop viruspmtv in potatoes growing at. It is confined to areas with a cool and humid climate and is found in northern europe, northern america, china, japan and the andes region. Sensitivity of potato cultivars to potato moptop virusinduced tuber necrosis o. When repeating a test you can use a previous file as a template by clicking open then selecting file name files of type experiment file as template international units no international units potato moptop virus 8 genesig easy kit handbook hb10. Pdf first report of potato moptop virus infecting potatoes in alberta. Potato mop top virus pmtv causes spraing, necrotic arcs or flecks in the flesh of tubers. Tobacco rattle virus trv and potato mop top virus pmtv are important diseases of potato that are difficult to diagnose reliably by visual symptoms. Pmtv belongs to family of virgaviridae, and is the type member of the genus pomovirus potato mop top virus.
Rotylenchulus reni ormis ditylenchus dipsaci ditylenchus. The virus is transmitted by the soilborne fungus which causes powdery scab spongospora subterranea, and can only survive longterm in the potato plant or in the fungus. Pdf potato moptop virus pmtv is a member of the genus pomovirus and has a singlestranded positivesense rna genome divided into three segments. Plants may be stunted with closely bunched upper leaves with wavy or rolled margins hence mop top. It is not a food safety issue and people can trust potatoes to eat. Unlike many of the important potato viruses, pmtv is not transmitted by aphids or nematodes. Total rna extracted from these tubers were tested by rtpcr at aberdeen, idaho, with primers specific for potato mop top virus pmtv 1 and tobacco rattle virus trv 4. Potato solanum tuberosumpotato moptop virus pacific. Wisconsin department of agriculture, trade and consumer. Gudmestad department of plant pathology, north dakota state university. Introduction sss has a narrower host range, infecting members of families potato moptop virus pmtv belongs to family virgaviridaae and solanaceae.
Northwestern pakistan is the leading seed potatoproducing area of the country. Chemical treatments for field application against viruses are currently not. The disease, potato mop top, is caused by the virus, potato mop top virus pmtv. This virus differs from the other major potato viruses in that it is transmitted by the. Pmtv is one of the causes of spraing disease, the other being tobacco rattle virus see below. Potato cultivars grown commercially in the region were screened against potato moptop virus pmtv under screenhouse conditions and in naturally infested fields during 20102014. The virus can be found in scab sporeballs and can remain viable in the field 18 years or longer without the host. Andean potato latent virus andean potato mottle virus amacacha virus b potato deforming mosaic vinis potato mop top virus potato spindle tuber viroid potato virus t potato yellow dwarf virus potato yellow vein virus tobacco rattle virus tobacco ringspot virus stolbur h o lasma globodcra spp. Pmtv belongs to family of virgaviridae, and is the type member of the genus pomovirus potato moptop virus. Sensitivity of potato cultivars to potato moptop virus. Seed potato certification is the process of assuring the quality of seed potatoes being marketed, usually done nationally according to regulated standards. Initially, one symptomatic and one asymptomatic tuber were tested. Powdery scab is very widely distributed but pmtv has a more narrow distribution. The behaviour of potato moptop virus in soil, and evidence for its transmission by spongospora subterranea wallr.
Potato moptop virus is a plant virus that largely affects potatoes and its a notifiable and unwanted organism in new zealand under the biosecurity act. Distribution and diversity of potato mop top virus in sweden abstract during the last decades, spraing disease caused by potato mop top virus pmtv has become a severe problem in the potato production throughout the nordic countries. The importance of potato mop top virus pmtv in scottish. Once established in fields, powdery scab can survive for up to 18 years in the absence of a potato crop. Also, the two viruses have different effects on chenopodium amaranticolor, and potato mop top virus seems not to infect phaseolus vulgaris. Secondary symptoms, on a plant developing from an infected tuber, can show yellow spots or patches on lower leaves and yellow or pale green v shapes on upper leaflets especially under cooler conditions. The potato cultivars, asterix, cardinal, desiree, diamant, kuroda, paramount, raja, rocco, sarpomira, and ultimas, were grown in. Detection of potato mop top virus furovirus on potato in. Analysis of potato mop top virus survival probability in postharvest storage s. Cause the potato mop top virus pmtv is a pomovirus vectored by the soilborne organism, spongospora subterrenea, which causes powdery scab of potato see potato powdery scab. Gudmestad, phytopathology 2019 rtqpcr and droplet digital rtddpcr assays of the total rna extracted directly from soil to detect pmtv both assays detected pmtv from all soil types used in samples with less.
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